Part:BBa_K260001:Design

pCC2FosMB

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1738
  Illegal EcoRI site found at 2324
  Illegal EcoRI site found at 3809
  Illegal EcoRI site found at 4889
  Illegal EcoRI site found at 14477
  Illegal EcoRI site found at 21238
  Illegal EcoRI site found at 30506
  Illegal XbaI site found at 7175
  Illegal XbaI site found at 10053
  Illegal XbaI site found at 14642
  Illegal XbaI site found at 14930
  Illegal XbaI site found at 17884
  Illegal XbaI site found at 17915
  Illegal XbaI site found at 20234
  Illegal SpeI site found at 2573
  Illegal SpeI site found at 28757
  Illegal PstI site found at 1836
  Illegal PstI site found at 1968
  Illegal PstI site found at 2697
  Illegal PstI site found at 4323
  Illegal PstI site found at 9689
  Illegal PstI site found at 10032
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1738
  Illegal EcoRI site found at 2324
  Illegal EcoRI site found at 3809
  Illegal EcoRI site found at 4889
  Illegal EcoRI site found at 14477
  Illegal EcoRI site found at 21238
  Illegal EcoRI site found at 30506
  Illegal NheI site found at 17298
  Illegal NheI site found at 48089
  Illegal SpeI site found at 2
  Illegal SpeI site found at 2573
  Illegal SpeI site found at 28757
  Illegal PstI site found at 16
  Illegal PstI site found at 1836
  Illegal PstI site found at 1968
  Illegal PstI site found at 2697
  Illegal PstI site found at 4323
  Illegal PstI site found at 9689
  Illegal PstI site found at 10032
  Illegal NotI site found at 9
  Illegal NotI site found at 22677
  Illegal NotI site found at 30175
  Illegal NotI site found at 48157
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1738
  Illegal EcoRI site found at 2324
  Illegal EcoRI site found at 3809
  Illegal EcoRI site found at 4889
  Illegal EcoRI site found at 14477
  Illegal EcoRI site found at 21238
  Illegal EcoRI site found at 30506
  Illegal BglII site found at 2335
  Illegal BglII site found at 25181
  Illegal BglII site found at 27248
  Illegal BglII site found at 37967
  Illegal BglII site found at 39393
  Illegal BglII site found at 41566
  Illegal BglII site found at 46713
  Illegal BamHI site found at 1870
  Illegal BamHI site found at 4280
  Illegal BamHI site found at 22400
  Illegal BamHI site found at 30527
  Illegal BamHI site found at 31427
  Illegal BamHI site found at 31887
  Illegal BamHI site found at 47143
  Illegal XhoI site found at 4046
  Illegal XhoI site found at 31812
  Illegal XhoI site found at 36045
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 48151
  Illegal suffix found at 2
  Illegal EcoRI site found at 1738
  Illegal EcoRI site found at 2324
  Illegal EcoRI site found at 3809
  Illegal EcoRI site found at 4889
  Illegal EcoRI site found at 14477
  Illegal EcoRI site found at 21238
  Illegal EcoRI site found at 30506
  Illegal XbaI site found at 7175
  Illegal XbaI site found at 10053
  Illegal XbaI site found at 14642
  Illegal XbaI site found at 14930
  Illegal XbaI site found at 17884
  Illegal XbaI site found at 17915
  Illegal XbaI site found at 20234
  Illegal SpeI site found at 2573
  Illegal SpeI site found at 28757
  Illegal PstI site found at 1836
  Illegal PstI site found at 1968
  Illegal PstI site found at 2697
  Illegal PstI site found at 4323
  Illegal PstI site found at 9689
  Illegal PstI site found at 10032
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 48151
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1738
  Illegal EcoRI site found at 2324
  Illegal EcoRI site found at 3809
  Illegal EcoRI site found at 4889
  Illegal EcoRI site found at 14477
  Illegal EcoRI site found at 21238
  Illegal EcoRI site found at 30506
  Illegal XbaI site found at 7175
  Illegal XbaI site found at 10053
  Illegal XbaI site found at 14642
  Illegal XbaI site found at 14930
  Illegal XbaI site found at 17884
  Illegal XbaI site found at 17915
  Illegal XbaI site found at 20234
  Illegal SpeI site found at 2
  Illegal SpeI site found at 2573
  Illegal SpeI site found at 28757
  Illegal PstI site found at 16
  Illegal PstI site found at 1836
  Illegal PstI site found at 1968
  Illegal PstI site found at 2697
  Illegal PstI site found at 4323
  Illegal PstI site found at 9689
  Illegal PstI site found at 10032
  Illegal NgoMIV site found at 12865
  Illegal NgoMIV site found at 13913
  Illegal NgoMIV site found at 24520
  Illegal NgoMIV site found at 24527
  Illegal NgoMIV site found at 24651
  Illegal NgoMIV site found at 25738
  Illegal NgoMIV site found at 29654
  Illegal AgeI site found at 8570
  Illegal AgeI site found at 13992
  Illegal AgeI site found at 25862
  Illegal AgeI site found at 27092
  Illegal AgeI site found at 27985
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 3602
  Illegal BsaI site found at 4043
  Illegal BsaI site found at 5783
  Illegal BsaI site found at 6346
  Illegal BsaI site found at 6934
  Illegal BsaI site found at 8864
  Illegal BsaI site found at 15098
  Illegal BsaI.rc site found at 675
  Illegal BsaI.rc site found at 1911
  Illegal BsaI.rc site found at 11284
  Illegal BsaI.rc site found at 11472
  Illegal BsaI.rc site found at 12748
  Illegal BsaI.rc site found at 14199
  Illegal BsaI.rc site found at 20602
  Illegal SapI site found at 6462
  Illegal SapI site found at 13800
  Illegal SapI site found at 16311
  Illegal SapI site found at 16333
  Illegal SapI site found at 26631
  Illegal SapI site found at 27841
  Illegal SapI.rc site found at 18502
  Illegal SapI.rc site found at 20759
  Illegal SapI.rc site found at 46761
  

Design Notes

The insertion site for BsdR was chosen to be upstream of MECP2 exon 1 but still with more than 10 kb of MECP genomic sequence upstream.

Source

Based on BBa_K260000, so it also contains human MECP2 genomic sequence, but has an insert taken from BBa_K260010.